rabbit il  (Bioss)

Journal: Scientific Reports

Article Title: IL-8 contributes to postoperative adhesion formation through the crosstalk of neutrophils and mesothelial cells

doi: 10.1038/s41598-026-42159-x

Figure Lengend Snippet: Evaluation of functional differences between IL-8 and CXCL1 in neutrophils. ( A ) Evaluation of IL-8 and CXCL1 activity in the U2OS-CXCR1 reporter cells ( left ) and CHO-K1-CXCR2 reporter cells ( right ). ( B ) Flow cytometric analysis of CXCR1 and CXCR2 surface expression on neutrophils. ( C ) Neutrophil chemotaxis assay toward IL-8 or CXCL1. RLU, relative luminescence units. Data in ( A ) and ( C ) are presented as the mean ± SD (n = 3).

Article Snippet: The concentrations of IL-8 in the tissue lysates were measured by sandwich immunoassay using two noncompetitive anti-human IL-8 antibodies (Chugai Pharmaceutical Co., Ltd.).

Techniques: Functional Assay, Activity Assay, Expressing, Chemotaxis Assay

Journal: Scientific Reports

Article Title: IL-8 contributes to postoperative adhesion formation through the crosstalk of neutrophils and mesothelial cells

doi: 10.1038/s41598-026-42159-x

Figure Lengend Snippet: Reciprocal amplification of IL-8 and TNF-α in MeT-5A cells. ( A , B ) Flow cytometric analysis of CXCR1 ( A ) and CXCR2 ( B ) surface expression on MeT-5A cells. Left: representative overlaid histograms. Right: quantification of MFI across biological replicates. P values were determined using Student’s t test. *** indicates P < 0.001. ( C ) Evaluation of TNF-α concentrations in culture medium. P values were determined using Tukey’s multiple comparison test. *** indicates P < 0.001. ( D ) Evaluation of IL-8 concentrations in culture medium. P values were determined using Williams’ test. * indicates P < 0.025. Quantitative data are presented as individual data points with mean ± SD. (n = 3).

Article Snippet: The concentrations of IL-8 in the tissue lysates were measured by sandwich immunoassay using two noncompetitive anti-human IL-8 antibodies (Chugai Pharmaceutical Co., Ltd.).

Techniques: Amplification, Expressing, Comparison

Journal: Scientific Reports

Article Title: IL-8 contributes to postoperative adhesion formation through the crosstalk of neutrophils and mesothelial cells

doi: 10.1038/s41598-026-42159-x

Figure Lengend Snippet: Fibrotic reactions in neutrophils and MeT-5A cells. ( A ) RNA expression change of TGFB1 in neutrophils stimulated with IL-8, IL-8 + AMY109, or TNF-α. ( B ) RNA expression change of TGFB1 , COL1A1 , FN1 , CCN2 ( CTGF ), SERPINE1 (PAI-1) and VIM in MeT-5A cells stimulated with TGF-β1 (0.4, 2, 10 ng/mL), TNF-α or IL-8. Data are presented as individual data points with mean ± SD. (n = 3) P values were determined using Dunnett’s test. * and *** indicate P < 0.05 and P < 0.001, respectively.

Article Snippet: The concentrations of IL-8 in the tissue lysates were measured by sandwich immunoassay using two noncompetitive anti-human IL-8 antibodies (Chugai Pharmaceutical Co., Ltd.).

Techniques: RNA Expression

Journal: Scientific Reports

Article Title: IL-8 contributes to postoperative adhesion formation through the crosstalk of neutrophils and mesothelial cells

doi: 10.1038/s41598-026-42159-x

Figure Lengend Snippet: Evaluation of IL-8 protein expression in injured abdominal walls from monkeys following cesarean sections (C-sections) and in postoperative adhesions (PA) model. ( A ) IL-8 concentrations in injured abdominal walls from monkeys following C-sections. Data are presented as individual data points with mean values indicated (n = 10 lesions for 0 h, 2 lesions for 6 h, Day 1, 3, 7, and 1 month). ( B ) IL-8 concentrations in injured abdominal walls from monkey PA models. Data are presented as individual data points with mean ± SD. (n = 6 lesions for 0 h, 8 lesions for 6 h, 4 lesions for Day 7) P values were determined using Steel’s test. * and ** indicate P < 0.05 and P < 0.01, respectively.

Article Snippet: The concentrations of IL-8 in the tissue lysates were measured by sandwich immunoassay using two noncompetitive anti-human IL-8 antibodies (Chugai Pharmaceutical Co., Ltd.).

Techniques: Expressing

Journal: Scientific Reports

Article Title: IL-8 contributes to postoperative adhesion formation through the crosstalk of neutrophils and mesothelial cells

doi: 10.1038/s41598-026-42159-x

Figure Lengend Snippet: Possible role of IL-8 and AMY109 in postoperative adhesion formation. IL-8 promotes neutrophil infiltration and forms a reciprocal amplification with TNF-α in mesothelial cells. Elevated TNF-α upregulates TGF-β1 in neutrophils, driving adhesion formation. Neutralization of IL-8 by AMY109 blocks neutrophil recruitment and subsequent inflammatory responses and fibrotic reactions, thereby preventing adhesion formation.

Article Snippet: The concentrations of IL-8 in the tissue lysates were measured by sandwich immunoassay using two noncompetitive anti-human IL-8 antibodies (Chugai Pharmaceutical Co., Ltd.).

Techniques: Amplification, Neutralization